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Which Type of Flask Should You Choose for Different Cell Lines? Crystal Has The Answer.

Views: 5 Author: vivi Publish Time: 2025-06-05 Origin: Site

In suspension cell culture experiments, different cell types have unique requirements in terms of oxygen exchange, shear force, surface treatment, and environmental conditions. Selecting the right type of culture flask is essential for maximizing cell viability and yield.

Here’s a breakdown of flask recommendations by cell type and usage tips:

Property	Recommended Flask Type	Notes
Growth Pattern	Suspension or adapted from adherent	Often requires adaptation
Container	Round-bottom polycarbonate flask with vent cap (500mL~2L)	Excellent gas exchange, reduces cell clumping
Special Tip	Round bottom + 0.2μm vent filter cap	Promotes aeration and prevents contamination
Common Uses	Protein expression, virus packaging, antibody production

Property	Recommended Flask Type	Notes
Culture Method	Suspension with shaker or bioreactor
Container	Round-bottom PETG or PC flasks (250mL~5L)	Maximizes gas-liquid contact surface
Special Tip	High transparency body + vent membrane cap	Easy observation, sufficient gas exchange
Culture Temp	27°C, no CO₂ required	Ideal for shaker environment
Common Uses	Recombinant protein expression, viral vector production

Property	Recommended Flask Type	Notes
Culture Phase	From adherent to suspension (e.g., microcarrier)	Expansion & scale-up
Container	High-aeration round-bottom flask (e.g., 2L with graduation)	Compatible with microcarriers or 3D culture
Special Tip	High gas permeability, requires accurate temp/gas control	Recommended with IncuShaker platform
Common Uses	Large-scale culture for stem cell drugs or exosome research

Property	Recommended Flask Type	Notes
Cell Features	Slow-growing, high nutrient demand	High oxygenation is essential
Container	Round-bottom flask with vent cap (>1L)	Reduces need for frequent feeding
Special Tip	Use low-shear shaker platform	Prevents cell damage
Common Uses	Antibody production, media optimization studies

Property	Recommended Flask Type	Notes
Growth Rate	Fast growth, high foam	Anti-foam caps recommended
Container	Wide-mouth round-bottom flask with vent membrane cap	Maximizes gas-liquid interface
Special Tip	High-speed shaking requires anti-slip flask holders
Common Uses	Protein expression, induction experiments, strain screening

Capacity ≠ Working Volume: Fill only 20–30% of total flask volume.
Choose the Right Cap: Vent filter caps are ideal for long-term culture — breathable and contamination-resistant.
Transparency Matters: PETG is highly transparent — ideal for visual cell monitoring.
Bottom Design Affects Mixing: Round-bottom for optimal mixing; flat-bottom for static culture.
Shaker Compatibility: Ensure flasks match shaker platform size and speed to prevent slipping or excessive shear stress.